Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RAD21

Cell type

Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

source_name
HepG2
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
hepatocellular carcinoma
antibody antibodydescription
Rabbit polyclonal. Antibody Target: RAD21
antibody targetdescription
Synthetic peptide (Human) conjugated to KLH - which represents a portion of human Rad21 encoded within exon 14
antibody vendorname
abcam
antibody vendorid
ab992
controlid
SL1781
labexpid
SL3586,SL3179
replicate
1,2
softwareversion
MACS
antibody antibodydescription
Rabbit polyclonal
antibody targetdescription
Synthetic peptide (Human) conjugated to KLH - which represents a portion of human Rad21 encoded within exon 14
antibody vendorname
Abcam
antibody vendorid
ab992
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.1
protocol description
2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)
controlid
SL1781
labexpid
SL3179
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803517: HudsonAlpha_ChipSeq_HepG2_Rad21_v041610.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
68324429
Reads aligned (%)
65.6
Duplicates removed (%)
26.9
Number of peaks
69944 (qval < 1E-05)

hg38

Number of total reads
68324429
Reads aligned (%)
66.6
Duplicates removed (%)
26.2
Number of peaks
70175 (qval < 1E-05)

Base call quality data from DBCLS SRA