Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BCLAF1

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Mouse monoclonal IgG, raised against a synthetic peptide corresponding to a.a. 900-909 of BCLAF1 of human origin. Antibody Target: BCLAF1
antibody targetdescription
This gene encodes a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins (RefSeq).
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-101388
protocol description
one 15-cycle round of PCR (Myers)
controlid
SL2455
labexpid
SL1273,SL1512
replicate
1,2
softwareversion
MACS
antibody antibodydescription
Mouse monoclonal IgG, raised against a synthetic peptide corresponding to a.a. 900-909 of BCLAF1 of human origin.
antibody targetdescription
This gene encodes a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins (RefSeq).
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-101388
treatment
None
treatment description
No special treatment or protocol applies
protocol
PCR1x
protocol description
1-cycle of PCR (Myers)
controlid
SL2455
labexpid
SL1273
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803515: HudsonAlpha_ChipSeq_K562_BCLAF1_(SC-101388)_PCR1x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
41869679
Reads aligned (%)
75.2
Duplicates removed (%)
12.3
Number of peaks
4692 (qval < 1E-05)

hg38

Number of total reads
41869679
Reads aligned (%)
75.9
Duplicates removed (%)
11.9
Number of peaks
4700 (qval < 1E-05)

Base call quality data from DBCLS SRA