Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GATA3

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
T-47D
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
epithelial cell line derived from a mammary ductal carcinoma.
antibody antibodydescription
Mouse monoclonal IgG raised against human recombinant GATA-3. Antibody Target: GATA3
antibody targetdescription
This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is a n important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (provided by RefSeq).
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-268
treatment description
1 h with 0.02% Dimethyl sufloxide (DMSO) (Myers)
controlid
SL1881
labexpid
SL2636,SL3013
replicate
1,2
softwareversion
MACS
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-268
treatment
DMSO_0.02pct
treatment description
1 h with 0.02% DMSO (Myers)
protocol
v041610.2
protocol description
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
controlid
SL1881
labexpid
SL2636
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803514: HudsonAlpha_ChipSeq_T-47D_GATA3_(SC-268)_DMSO_0.02pct_v041610.2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
64222464
Reads aligned (%)
82.1
Duplicates removed (%)
9.0
Number of peaks
64405 (qval < 1E-05)

hg38

Number of total reads
64222464
Reads aligned (%)
84.1
Duplicates removed (%)
7.5
Number of peaks
64696 (qval < 1E-05)

Base call quality data from DBCLS SRA