Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BCLAF1

Cell type

Cell type Class
Blood
Cell type
GM12878
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Attributes by original data submitter

Sample

source_name
GM12878
biomaterial_provider
GM12878
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus
antibody antibodydescription
Mouse monoclonal IgG, raised against a synthetic peptide corresponding to a.a. 900-909 of BCLAF1 of human origin. Antibody Target: BCLAF1
antibody targetdescription
This gene encodes a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins (RefSeq).
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-101388
controlid
SL1613,SL1395
labexpid
SL1509,SL2128
replicate
1,2
softwareversion
MACS
antibody antibodydescription
Mouse monoclonal IgG, raised against a synthetic peptide corresponding to a.a. 900-909 of BCLAF1 of human origin.
antibody targetdescription
This gene encodes a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins (RefSeq).
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-101388
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.1
protocol description
2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)
controlid
SL1395
labexpid
SL1509
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803509: HudsonAlpha_ChipSeq_GM12878_BCLAF1_(SC-101388)_v041610.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
68774686
Reads aligned (%)
81.9
Duplicates removed (%)
6.5
Number of peaks
39979 (qval < 1E-05)

hg38

Number of total reads
68774686
Reads aligned (%)
83.3
Duplicates removed (%)
6.7
Number of peaks
39124 (qval < 1E-05)

Base call quality data from DBCLS SRA