Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RXRA

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
H1-hESC
biomaterial_provider
WiCell Research Institute
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
RXRa(D-20) is an affinity purified rabbit polyclonal antibody raised against a peptide mapping at the N-terminus of RXRalpha of human origin. Recommended for detection of RXRalpha of m, r and h origin by WB, IP, IF, IHC(P) and ELISA. Antibody Target: RXRA
antibody targetdescription
Retinoid X receptors (RXRs) and retinoic acid receptors (RARs), are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. These receptors exert their action by binding, as homodimers or heterodimers, to specific sequences in the promoters of target genes and regulating their transcription. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcriptional regulators.
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-553
controlid
SL1398
labexpid
SL1163,SL2346
replicate
1,2
cell sex
M
antibody
RXRA
antibody antibodydescription
RXRa(D-20) is an affinity purified rabbit polyclonal antibody raised against a peptide mapping at the N-terminus of RXRalpha of human origin. Recommended for detection of RXRalpha of m, r and h origin by WB, IP, IF, IHC(P) and ELISA.
antibody targetdescription
Retinoid X receptors (RXRs) and retinoic acid receptors (RARs), are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. These receptors exert their action by binding, as homodimers or heterodimers, to specific sequences in the promoters of target genes and regulating their transcription. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcriptional regulators.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-553
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.2
protocol description
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
controlid
SL1398
labexpid
SL1163
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803506: HudsonAlpha_ChipSeq_H1-hESC_RXRA_v041610.2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
37434149
Reads aligned (%)
63.0
Duplicates removed (%)
19.6
Number of peaks
1591 (qval < 1E-05)

hg38

Number of total reads
37434149
Reads aligned (%)
64.2
Duplicates removed (%)
18.5
Number of peaks
2472 (qval < 1E-05)

Base call quality data from DBCLS SRA