Antigen

Antigen Class

TFs and others

Antigen

ZBTB33

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

K562

biomaterial_provider

ATCC

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

antibody antibodydescription

Kaiso (6F8) Mouse monoclonal IgG1. Raised against amino acids 1-504 representing full length Kaiso of human origin. Recommended for detection of Kaiso of m, r, h, canine and chicken origin by WB, IP, IF and ELISA. Antibody Target: ZBTB33

antibody targetdescription

KAISO is a transcriptional regulator that binds, via its zinc fingers, to DNA sequences containing methylated CGCG or to the consensus KAISO-binding site (KBS) TCCTGCNA

antibody vendorname

Santa Cruz Biotechnology

antibody vendorid

sc-23871

protocol description

one 15-cycle round of PCR (Myers)

controlid

SL2455

labexpid

SL1114,SL1320

replicate

1,2

softwareversion

MACS

antibody antibodydescription

Kaiso (6F8) Mouse monoclonal IgG1. Raised against amino acids 1-504 representing full length Kaiso of human origin. Recommended for detection of Kaiso of m, r, h, canine and chicken origin by WB, IP, IF and ELISA

antibody targetdescription

KAISO is a transcriptional regulator that binds, via its zinc fingers, to DNA sequences containing methylated CGCG or to the consensus KAISO-binding site (KBS) TCCTGCNA

antibody vendorname

Santa Cruz Biotech

antibody vendorid

sc-23871

treatment

None

treatment description

No special treatment or protocol applies

protocol

PCR1x

protocol description

1-cycle of PCR (Myers)

controlid

SL2455

labexpid

SL1114

replicate

1

replicate description

tier 1

Sequenced DNA Library

library_name

GSM803504: HudsonAlpha_ChipSeq_K562_ZBTB33_PCR1x

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

54114620

Reads aligned (%)

89.1

Duplicates removed (%)

13.3

Number of peaks

3543 (qval < 1E-05)

hg19

Number of total reads

54114620

Reads aligned (%)

88.6

Duplicates removed (%)

14.5

Number of peaks

3816 (qval < 1E-05)