Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ZBTB33

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Kaiso (6F8) Mouse monoclonal IgG1. Raised against amino acids 1-504 representing full length Kaiso of human origin. Recommended for detection of Kaiso of m, r, h, canine and chicken origin by WB, IP, IF and ELISA. Antibody Target: ZBTB33
antibody targetdescription
KAISO is a transcriptional regulator that binds, via its zinc fingers, to DNA sequences containing methylated CGCG or to the consensus KAISO-binding site (KBS) TCCTGCNA
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-23871
protocol description
one 15-cycle round of PCR (Myers)
controlid
SL2455
labexpid
SL1114,SL1320
replicate
1,2
softwareversion
MACS
antibody antibodydescription
Kaiso (6F8) Mouse monoclonal IgG1. Raised against amino acids 1-504 representing full length Kaiso of human origin. Recommended for detection of Kaiso of m, r, h, canine and chicken origin by WB, IP, IF and ELISA
antibody targetdescription
KAISO is a transcriptional regulator that binds, via its zinc fingers, to DNA sequences containing methylated CGCG or to the consensus KAISO-binding site (KBS) TCCTGCNA
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-23871
treatment
None
treatment description
No special treatment or protocol applies
protocol
PCR1x
protocol description
1-cycle of PCR (Myers)
controlid
SL2455
labexpid
SL1114
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803504: HudsonAlpha_ChipSeq_K562_ZBTB33_PCR1x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
54114620
Reads aligned (%)
75.7
Duplicates removed (%)
14.5
Number of peaks
3816 (qval < 1E-05)

hg38

Number of total reads
54114620
Reads aligned (%)
77.3
Duplicates removed (%)
13.3
Number of peaks
3543 (qval < 1E-05)

Base call quality data from DBCLS SRA