Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SRF

Cell type

Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

source_name
HepG2
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
hepatocellular carcinoma
antibody antibodydescription
Rabbit polyclonal. Antibody Target: SRF
antibody targetdescription
Serum response transcription factor
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-335
controlid
SL1781
labexpid
SL3275,SL3156
replicate
1,2
softwareversion
MACS
antibody antibodydescription
Rabbit polyclonal
antibody targetdescription
Serum response transcription factor
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-335
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.1
protocol description
2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)
controlid
SL1781
labexpid
SL3156
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803502: HudsonAlpha_ChipSeq_HepG2_SRF_v041610.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
67555124
Reads aligned (%)
68.2
Duplicates removed (%)
23.8
Number of peaks
8524 (qval < 1E-05)

hg38

Number of total reads
67555124
Reads aligned (%)
69.7
Duplicates removed (%)
22.6
Number of peaks
8485 (qval < 1E-05)

Base call quality data from DBCLS SRA