Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EP300

Cell type

Cell type Class
Neural
Cell type
SK-N-SH
Primary Tissue
Brain
Tissue Diagnosis
Neuroblastoma

Attributes by original data submitter

Sample

source_name
SK-N-SH_RA
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
neuroblastoma cell line, treatment: differentiated with retinoic acid, (Biedler, et al. Morphology and Growth, Tumorigenicity, and Cytogenetics of Human Neuroblastoma Cells in Continuous Culture. Cancer Research 33, 2643-2652, November 1973.)
antibody antibodydescription
Rabbit Polyclonal. Antibody Target: P300
antibody targetdescription
EP300(c-20)
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-585
controlid
SL2905
labexpid
SL2918,SL2910
replicate
1,2
softwareversion
MACS
antibody antibodydescription
Rabbit Polyclonal
antibody targetdescription
EP300(c-20)
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-585
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.2
protocol description
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
controlid
SL2905
labexpid
SL2910
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803495: HudsonAlpha_ChipSeq_SK-N-SH_RA_p300_v041610.2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
48740291
Reads aligned (%)
67.5
Duplicates removed (%)
20.2
Number of peaks
80683 (qval < 1E-05)

hg38

Number of total reads
48740291
Reads aligned (%)
68.8
Duplicates removed (%)
19.2
Number of peaks
80819 (qval < 1E-05)

Base call quality data from DBCLS SRA