Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
HDAC2

Cell type

Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

source_name
HepG2
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
hepatocellular carcinoma
antibody antibodydescription
Goat polyclonal IgG, epitope mapping at the C-terminus of HDAC2 of human origin. Antibody Target: HDAC2
antibody targetdescription
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. (provided by RefSeq)
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-6296
controlid
SL1781
labexpid
SL3177,SL3583
replicate
1,2
softwareversion
MACS
antibody antibodydescription
Goat polyclonal IgG, epitope mapping at the C-terminus of HDAC2 of human origin.
antibody targetdescription
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. (provided by RefSeq)
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-6296
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.1
protocol description
2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)
controlid
SL1781
labexpid
SL3177
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803493: HudsonAlpha_ChipSeq_HepG2_HDAC2_(SC-6296)_v041610.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
69837964
Reads aligned (%)
63.5
Duplicates removed (%)
28.1
Number of peaks
35054 (qval < 1E-05)

hg38

Number of total reads
69837964
Reads aligned (%)
64.6
Duplicates removed (%)
27.3
Number of peaks
35312 (qval < 1E-05)

Base call quality data from DBCLS SRA