Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TAF1

Cell type

Cell type Class
Blood
Cell type
GM12892
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstein-Barr Virus transformed

Attributes by original data submitter

Sample

source_name
GM12892
biomaterial_provider
Coriell
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstein-Barr Virus transformed
antibody antibodydescription
Mouse monoclonal (p250). Antibody Target: TAF1
antibody targetdescription
TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa. Also designated TAF250.
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-735
controlid
SL818
labexpid
SL736,SL945
replicate
1,2
antibody
TAF1
antibody antibodydescription
Mouse monoclonal (p250)
antibody targetdescription
TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa. Also designated TAF250.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-735
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.2
protocol description
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
controlid
SL818
labexpid
SL736
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803489: HudsonAlpha_ChipSeq_GM12892_TAF1_v041610.2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
61329129
Reads aligned (%)
59.5
Duplicates removed (%)
8.3
Number of peaks
11072 (qval < 1E-05)

hg38

Number of total reads
61329129
Reads aligned (%)
61.4
Duplicates removed (%)
7.7
Number of peaks
10580 (qval < 1E-05)

Base call quality data from DBCLS SRA