SRX100529
GSM803484: HudsonAlpha ChipSeq H1-hESC Pol2-4H8 v041610.2

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
H1-hESC
biomaterial_provider
WiCell Research Institute
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Mouse monoclonal to RNA polymerase II CTD repeat YSPTSPS antibody (4H8) - ChIP Grade. Antibody Target: POL2
antibody targetdescription
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes
antibody vendorname
abcam
antibody vendorid
ab5408
controlid
SL969
labexpid
SL979,SL1051
replicate
1,2
cell sex
M
antibody
Pol2-4H8
antibody antibodydescription
Mouse monoclonal to RNA polymerase II CTD repeat YSPTSPS antibody (4H8) - ChIP Grade
antibody targetdescription
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes
antibody vendorname
Abcam
antibody vendorid
ab5408
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.2
protocol description
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
controlid
SL969
labexpid
SL979
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803484: HudsonAlpha_ChipSeq_H1-hESC_Pol2-4H8_v041610.2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

Where can I get the processing logs?

Read processing pipeline log

hg38

Number of total reads
74085847
Reads aligned (%)
63.5
Duplicates removed (%)
12.8
Number of peaks
49127 (qval < 1E-05)

hg19

Number of total reads
74085847
Reads aligned (%)
63.2
Duplicates removed (%)
13.0
Number of peaks
49031 (qval < 1E-05)

Base call quality data from DBCLS SRA