Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BHLHE40

Cell type

Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

source_name
HepG2
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
hepatocellular carcinoma
antibody antibodydescription
Mouse monoclonal. Dec-1(S-8) is an IgG2b antibody raised against recombinant DEC1 of human origin. Recommended for detection of DEC1 of mouse and human origin by WB, IP and ELISA. Antibody Target: BHLHE40
antibody targetdescription
This gene encodes a basic helix-loop-helix protein expressed in various tissues. Expression in the chondrocytes is responsive to the addition of Bt2cAMP. The encoded protein is believed to be involved in the control of cell differe
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-101023
controlid
SL1781,SL593
labexpid
SL3460,SL1059
replicate
1,2
softwareversion
MACS
antibody antibodydescription
Dec-1(S-8) is a mouse monoclonal IgG2b antibody raised against recombinant DEC1 of human origin. Recommended for detection of DEC1 of m and h origin by WB, IP and ELISA.
antibody targetdescription
This gene encodes a basic helix-loop-helix protein expressed in various tissues. Expression in the chondrocytes is responsive to the addition of Bt2cAMP. The encoded protein is believed to be involved in the control of cell differe
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-101023
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.1
protocol description
2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)
controlid
SL593
labexpid
SL1059
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803483: HudsonAlpha_ChipSeq_HepG2_BHLHE40_v041610.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
58177266
Reads aligned (%)
56.6
Duplicates removed (%)
33.6
Number of peaks
2947 (qval < 1E-05)

hg38

Number of total reads
58177266
Reads aligned (%)
58.2
Duplicates removed (%)
32.2
Number of peaks
3530 (qval < 1E-05)

Base call quality data from DBCLS SRA