Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FOXP2

Cell type

Cell type Class
Neural
Cell type
PFSK-1
Primary Tissue
Brain
Tissue Diagnosis
Medulloblastoma

Attributes by original data submitter

Sample

source_name
PFSK-1
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
neuroectodermal cell line derived from a cerebral brain tumor, (PMID: 1316433)
antibody antibodydescription
Rabbit polyclonal, raised against C-terminal 127 amino acids of FOXP2 of human origin. Antibody Target: FOXP2
antibody targetdescription
This gene encodes a member of the forkhead/winged-helix (FOX) family of transcription factors. Transcriptional repressor, involved in the development of speech
antibody vendorname
abcam
antibody vendorid
ab16046
protocol description
a 25-cycle round of PCR and an additional 15-cycle round of PCR after gel size selection (Myers)
controlid
SL16
labexpid
SL14,SL13
replicate
1,2
antibody antibodydescription
rabbit polyclonal
antibody targetdescription
transcriptional repressor, involved in development of speech
antibody vendorname
Abcam
antibody vendorid
ab16046
treatment
None
treatment description
No special treatment or protocol applies
protocol
PCR2x
protocol description
2-cycles of PCR (Myers)
controlid
SL16
labexpid
SL13
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803480: HudsonAlpha_ChipSeq_PFSK-1_FOXP2_PCR2x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
6477034
Reads aligned (%)
85.1
Duplicates removed (%)
3.3
Number of peaks
10492 (qval < 1E-05)

hg38

Number of total reads
6477034
Reads aligned (%)
85.4
Duplicates removed (%)
3.3
Number of peaks
10497 (qval < 1E-05)

Base call quality data from DBCLS SRA