Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
REST

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa-S3
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell lineage
ectoderm
antibody antibodydescription
Mouse monoclonal. Antibody Target: NRSF
antibody targetdescription
Neuron-restrictive silencer transcription factor
antibody vendorname
Dr. David Anderson at Caltech
antibody vendorid
(Made by Protein Expression Center: pec@caltech.edu)
protocol description
one 15-cycle round of PCR (Myers)
controlid
SL592
labexpid
SL679,SL1322
replicate
1,2
softwareversion
MACS
antibody vendorname
David Anderson, Caltech
antibody vendorid
(Made by Protein Expression Center: pec@caltech.edu)
treatment
None
treatment description
No special treatment or protocol applies
protocol
PCR1x
protocol description
1-cycle of PCR (Myers)
controlid
SL592
labexpid
SL679
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803478: HudsonAlpha_ChipSeq_HeLa-S3_NRSF_PCR1x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
56675123
Reads aligned (%)
52.8
Duplicates removed (%)
17.3
Number of peaks
12874 (qval < 1E-05)

hg38

Number of total reads
56675123
Reads aligned (%)
53.9
Duplicates removed (%)
16.4
Number of peaks
12399 (qval < 1E-05)

Base call quality data from DBCLS SRA