Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BCL11A

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
H1-hESC
biomaterial_provider
WiCell Research Institute
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Mouse monoclonal [18B12DE6] to Ctip1. Raised against a cocktail of BCL11A fusion proteins. Reacts with human and mouse. Tested for ICC/IF, IHC-P, IP, WB. Antibody Target: BCL11A
antibody targetdescription
This gene encodes a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. Functions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. An essential factor in lymphopoiesis,is required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1 (By similarity)
antibody vendorname
abcam
antibody vendorid
ab19489
controlid
SL1398
labexpid
SL3989
cell lineage
inner cell mass
cell sex
M
antibody
BCL11A
antibody antibodydescription
Mouse monoclonal [18B12DE6] to Ctip1. Raised against a cocktail of BCL11A fusion proteins. Reacts with human and mouse. Tested for ICC/IF, IHC-P, IP, WB
antibody targetdescription
This gene encodes a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. Functions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. An essential factor in lymphopoiesis,is required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1 (By similarity)
antibody vendorname
Abcam
antibody vendorid
ab19489
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.2
protocol description
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
controlid
SL1398
labexpid
SL3989
replicate
2
replicate description
tier 2

Sequenced DNA Library

library_name
GSM803476: HudsonAlpha_ChipSeq_H1-hESC_BCL11A_v041610.2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
19553179
Reads aligned (%)
78.1
Duplicates removed (%)
12.2
Number of peaks
1050 (qval < 1E-05)

hg38

Number of total reads
19553179
Reads aligned (%)
79.5
Duplicates removed (%)
11.2
Number of peaks
848 (qval < 1E-05)

Base call quality data from DBCLS SRA