Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ZBTB33

Cell type

Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
HCT-116
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Kaiso (6F8) Mouse monoclonal IgG1. Raised against amino acids 1-504 representing full length Kaiso of human origin. Recommended for detection of Kaiso of m, r, h, canine and chicken origin by WB, IP, IF and ELISA. Antibody Target: ZBTB33
antibody targetdescription
KAISO is a transcriptional regulator that binds, via its zinc fingers, to DNA sequences containing methylated CGCG or to the consensus KAISO-binding site (KBS) TCCTGCNA
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-23871
controlid
SL3457
labexpid
SL3970,SL3646
replicate
1,2
softwareversion
MACS
antibody
ZBTB33
antibody antibodydescription
Kaiso (6F8) Mouse monoclonal IgG1. Raised against amino acids 1-504 representing full length Kaiso of human origin. Recommended for detection of Kaiso of m, r, h, canine and chicken origin by WB, IP, IF and ELISA
antibody targetdescription
KAISO is a transcriptional regulator that binds, via its zinc fingers, to DNA sequences containing methylated CGCG or to the consensus KAISO-binding site (KBS) TCCTGCNA
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-23871
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.1
protocol description
2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)
controlid
SL3457
labexpid
SL3646
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803458: HudsonAlpha_ChipSeq_HCT-116_ZBTB33_v041610.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
44924278
Reads aligned (%)
69.2
Duplicates removed (%)
17.9
Number of peaks
4575 (qval < 1E-05)

hg38

Number of total reads
44924278
Reads aligned (%)
70.1
Duplicates removed (%)
17.2
Number of peaks
4519 (qval < 1E-05)

Base call quality data from DBCLS SRA