Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FOSL2

Cell type

Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

source_name
HepG2
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
hepatocellular carcinoma
antibody antibodydescription
Rabbit polyclonal. Fra-2(Q-20) is an affinity purified raised against the N-terminus of Fra-2 of human origin. Recommended for detection of Fra-2 of m, r, h and chicken origin by WB, IP, IF, IHC(P) and ELISA. Antibody Target: FOSL2
antibody targetdescription
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation.
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-604
controlid
SL819,SL593
labexpid
SL1107,SL632
labversion
SL632
replicate
1,2
softwareversion
MACS
antibody targetdescription
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-604
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.1
protocol description
2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)
controlid
SL593
labexpid
SL632
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803451: HudsonAlpha_ChipSeq_HepG2_FOSL2_v041610.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
81949090
Reads aligned (%)
60.1
Duplicates removed (%)
11.8
Number of peaks
44560 (qval < 1E-05)

hg38

Number of total reads
81949090
Reads aligned (%)
61.3
Duplicates removed (%)
10.7
Number of peaks
44741 (qval < 1E-05)

Base call quality data from DBCLS SRA