Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
HEY1

Cell type

Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

source_name
HepG2
biomaterial_provider
ATCC
lab
HudsonAlpha
lab description
Myers - Hudson Alpha Institute for Biotechnology
datatype
ChipSeq
datatype description
ChIP-Seq
cell
HepG2
cell organism
human
cell description
liver carcinoma
cell karyotype
cancer
cell lineage
endoderm
cell sex
M
antibody
HEY1
antibody antibodydescription
HRT1 (C-20) Goat polyclonal IgG, 200 ?g/ml. Epitope mapping near the C-terminus of HRT1 of human origin
antibody targetdescription
This gene encodes a nuclear protein belonging to the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcriptional repressors. Expression of this gene is induced by the Notch and c-Jun signal transduction pathways. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternative splicing results in multiple transcript variants.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-16424
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.1
protocol description
2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)
controlid
SL593
labexpid
SL1057
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803448: HudsonAlpha_ChipSeq_HepG2_HEY1_v041610.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
67635839
Reads aligned (%)
63.5
Duplicates removed (%)
18.6
Number of peaks
34710 (qval < 1E-05)

hg38

Number of total reads
67635839
Reads aligned (%)
64.4
Duplicates removed (%)
18.2
Number of peaks
34679 (qval < 1E-05)

Base call quality data from DBCLS SRA