Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Mouse monoclonal to RNA polymerase II CTD repeat YSPTSPS antibody (4H8) - ChIP Grade. Antibody Target: POL2
antibody targetdescription
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes
antibody vendorname
abcam
antibody vendorid
ab5408
controlid
SL2455
labexpid
SL2940,SL2939
replicate
1,2
softwareversion
MACS
antibody
Pol2-4H8
antibody antibodydescription
Mouse monoclonal to RNA polymerase II CTD repeat YSPTSPS antibody (4H8) - ChIP Grade
antibody targetdescription
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes
antibody vendorname
Abcam
antibody vendorid
ab5408
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.1
protocol description
2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)
controlid
SL2455
labexpid
SL2939
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803443: HudsonAlpha_ChipSeq_K562_Pol2-4H8_v041610.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
46172685
Reads aligned (%)
84.5
Duplicates removed (%)
21.2
Number of peaks
35401 (qval < 1E-05)

hg19

Number of total reads
46172685
Reads aligned (%)
84.0
Duplicates removed (%)
21.1
Number of peaks
35368 (qval < 1E-05)

Base call quality data from DBCLS SRA