Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
REST

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Mouse monoclonal. Antibody Target: NRSF
antibody targetdescription
Neuron-restrictive silencer transcription factor
antibody vendorname
Dr. David Anderson at Caltech
antibody vendorid
(Made by Protein Expression Center: pec@caltech.edu)
controlid
SL2455
labexpid
SL3821,SL3822
replicate
1,2
softwareversion
MACS
antibody
NRSF
antibody antibodydescription
Mouse monoclonal
antibody targetdescription
Neuron-restrictive silencer transcription factor
antibody vendorname
David Anderson, Caltech
antibody vendorid
(Made by Protein Expression Center: pec@caltech.edu)
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.2
protocol description
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
controlid
SL2455
labexpid
SL3821
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803440: HudsonAlpha_ChipSeq_K562_NRSF_v041610.2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
62975946
Reads aligned (%)
44.6
Duplicates removed (%)
38.7
Number of peaks
27443 (qval < 1E-05)

hg38

Number of total reads
62975946
Reads aligned (%)
45.1
Duplicates removed (%)
38.2
Number of peaks
27481 (qval < 1E-05)

Base call quality data from DBCLS SRA