Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
POU5F1

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
H1-hESC
biomaterial_provider
WiCell Research Institute
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Rabbit polyclonal IgG, epitope corresponding to amino acids 1-134 of Oct3/4 of human origin. Antibody Target: POU5F1
antibody targetdescription
This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12 (provided by RefSeq).
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-9081
controlid
SL1398
labexpid
SL2548,SL3453
replicate
1,2
softwareversion
MACS
antibody
POU5F1_(SC-9081)
antibody antibodydescription
Rabbit polyclonal IgG, epitope corresponding to amino acids 1-134 of Oct3/4 of human origin.
antibody targetdescription
This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12 (provided by RefSeq).
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-9081
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.2
protocol description
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
controlid
SL1398
labexpid
SL2548
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803438: HudsonAlpha_ChipSeq_H1-hESC_POU5F1_(SC-9081)_v041610.2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
53406766
Reads aligned (%)
63.2
Duplicates removed (%)
28.9
Number of peaks
2476 (qval < 1E-05)

hg38

Number of total reads
53406766
Reads aligned (%)
65.1
Duplicates removed (%)
27.3
Number of peaks
4490 (qval < 1E-05)

Base call quality data from DBCLS SRA