Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
A549
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
epithelial cell line derived from a lung carcinoma tissue. (PMID: 175022), "This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old caucasian male." - ATCC, newly promoted to tier 2: not in 2011 analysis
treatment description
1 h with 0.02% Ethanol (Myers)
protocol description
a 25-cycle round of PCR and an additional 15-cycle round of PCR after gel size selection (Myers)
controlid
SL307
labexpid
SL307
cell karyotype
cancer
cell lineage
endoderm
cell sex
M
antibody
RevXlinkChromatin
treatment
EtOH_0.02pct
treatment description
1 h with 0.02% EtOH (Myers)
protocol
PCR2x
protocol description
2-cycles of PCR (Myers)
controlid
SL307
labexpid
SL307
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803435: HudsonAlpha_ChipSeq_A549_RevXlinkChromatin_EtOH_0.02pct_PCR2x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
46609331
Reads aligned (%)
51.9
Duplicates removed (%)
2.3
Number of peaks
1673 (qval < 1E-05)

hg19

Number of total reads
46609331
Reads aligned (%)
51.5
Duplicates removed (%)
3.1
Number of peaks
1816 (qval < 1E-05)

Base call quality data from DBCLS SRA