Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TCF12

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
H1-hESC
biomaterial_provider
WiCell Research Institute
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
HEB (A-20) Rabbit polyclonal IgG, 200 ?g/ml. Epitope mapping within the C-terminus of HEB of human origin. Antibody Target: TCF12
antibody targetdescription
The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-357
protocol description
one 15-cycle round of PCR (Myers)
controlid
SL1398
labexpid
SL1158,SL1315
replicate
1,2
softwareversion
MACS
antibody antibodydescription
HEB (A-20) Rabbit polyclonal IgG, 200 ?g/ml. Epitope mapping within the C-terminus of HEB of human origin
antibody targetdescription
The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-357
treatment
None
treatment description
No special treatment or protocol applies
protocol
PCR1x
protocol description
1-cycle of PCR (Myers)
controlid
SL1398
labexpid
SL1158
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803427: HudsonAlpha_ChipSeq_H1-hESC_TCF12_PCR1x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
38844621
Reads aligned (%)
70.8
Duplicates removed (%)
13.5
Number of peaks
19566 (qval < 1E-05)

hg38

Number of total reads
38844621
Reads aligned (%)
72.1
Duplicates removed (%)
12.5
Number of peaks
19171 (qval < 1E-05)

Base call quality data from DBCLS SRA