Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SRF

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
H1-hESC
biomaterial_provider
WiCell Research Institute
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Rabbit polyclonal. Antibody Target: SRF
antibody targetdescription
Serum response transcription factor
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-335
protocol description
one 15-cycle round of PCR (Myers)
controlid
SL1398
labexpid
SL1483,SL1659
replicate
1,2
softwareversion
MACS
antibody antibodydescription
Rabbit polyclonal
antibody targetdescription
Serum response transcription factor
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-335
treatment
None
treatment description
No special treatment or protocol applies
protocol
PCR1x
protocol description
1-cycle of PCR (Myers)
controlid
SL1398
labexpid
SL1483
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803425: HudsonAlpha_ChipSeq_H1-hESC_SRF_PCR1x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
59629614
Reads aligned (%)
60.4
Duplicates removed (%)
23.5
Number of peaks
6939 (qval < 1E-05)

hg38

Number of total reads
59629614
Reads aligned (%)
61.6
Duplicates removed (%)
22.4
Number of peaks
6711 (qval < 1E-05)

Base call quality data from DBCLS SRA