Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Lung

Cell type

A549

Primary Tissue

Lung

Tissue Diagnosis

Carcinoma

Sample

source_name

A549

biomaterial_provider

ATCC

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell description

epithelial cell line derived from a lung carcinoma tissue. (PMID: 175022), "This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old caucasian male." - ATCC, newly promoted to tier 2: not in 2011 analysis

treatment description

1 h with 100 nM Dexamethasone (Myers)

protocol description

one 15-cycle round of PCR (Myers)

controlid

SL474

labexpid

SL474

cell karyotype

cancer

cell lineage

endoderm

cell sex

M

antibody

RevXlinkChromatin

treatment

DEX_100nM

treatment description

1 h with 100 nM DEX (Myers)

protocol

PCR1x

protocol description

1-cycle of PCR (Myers)

controlid

SL474

labexpid

SL474

replicate

1

replicate description

tier 1

Sequenced DNA Library

library_name

GSM803417: HudsonAlpha_ChipSeq_A549_RevXlinkChromatin_DEX_100nM_PCR1x

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

19508850

Reads aligned (%)

77.9

Duplicates removed (%)

2.3

Number of peaks

539 (qval < 1E-05)

hg19

Number of total reads

19508850

Reads aligned (%)

77.2

Duplicates removed (%)

3.1

Number of peaks

717 (qval < 1E-05)