Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RAD21

Cell type

Cell type Class
Blood
Cell type
GM12878
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Attributes by original data submitter

Sample

source_name
GM12878
biomaterial_provider
GM12878
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus
antibody antibodydescription
Rabbit polyclonal. Antibody Target: RAD21
antibody targetdescription
Synthetic peptide (Human) conjugated to KLH - which represents a portion of human Rad21 encoded within exon 14
antibody vendorname
abcam
antibody vendorid
ab992
controlid
SL1613
labexpid
SL3933,SL3934
replicate
1,2
softwareversion
MACS
antibody antibodydescription
Rabbit polyclonal
antibody targetdescription
Synthetic peptide (Human) conjugated to KLH - which represents a portion of human Rad21 encoded within exon 14
antibody vendorname
Abcam
antibody vendorid
ab992
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.1
protocol description
2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)
controlid
SL1613
labexpid
SL3933
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803416: HudsonAlpha_ChipSeq_GM12878_Rad21_v041610.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
45741895
Reads aligned (%)
56.3
Duplicates removed (%)
35.1
Number of peaks
47730 (qval < 1E-05)

hg38

Number of total reads
45741895
Reads aligned (%)
57.5
Duplicates removed (%)
34.5
Number of peaks
46025 (qval < 1E-05)

Base call quality data from DBCLS SRA