Antigen

Antigen Class

TFs and others

Antigen

ZEB1

Cell type

Cell type Class

Blood

Cell type

GM12878

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Sample

source_name

GM12878

biomaterial_provider

GM12878

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

antibody antibodydescription

Rabbit polyclonal IgG, epitope corresponding to amino acids 39-140 mapping near the N-terminus of ZEB1 of human origin. Antibody Target: ZEB1

antibody targetdescription

This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have ben associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described. (provided by RefSeq).

antibody vendorname

Santa Cruz Biotechnology

antibody vendorid

sc-25388

controlid

SL1394

labexpid

SL2957,SL3254

replicate

1,2

softwareversion

MACS

antibody antibodydescription

Rabbit polyclonal IgG, epitope corresponding to amino acids 39-140 mapping near the N-terminus of ZEB1 of human origin.

antibody targetdescription

This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have ben associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described. (provided by RefSeq).

antibody vendorname

Santa Cruz Biotech

antibody vendorid

sc-25388

treatment

None

treatment description

No special treatment or protocol applies

protocol

v041610.2

protocol description

2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)

controlid

SL1394

labexpid

SL2957

replicate

1

replicate description

tier 1

Sequenced DNA Library

library_name

GSM803411: HudsonAlpha_ChipSeq_GM12878_ZEB1_(SC-25388)_v041610.2

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

47924244

Reads aligned (%)

73.8

Duplicates removed (%)

43.9

Number of peaks

8099 (qval < 1E-05)

hg19

Number of total reads

47924244

Reads aligned (%)

71.9

Duplicates removed (%)

44.0

Number of peaks

8311 (qval < 1E-05)