Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ZEB1

Cell type

Cell type Class
Blood
Cell type
GM12878
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Attributes by original data submitter

Sample

source_name
GM12878
biomaterial_provider
GM12878
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus
antibody antibodydescription
Rabbit polyclonal IgG, epitope corresponding to amino acids 39-140 mapping near the N-terminus of ZEB1 of human origin. Antibody Target: ZEB1
antibody targetdescription
This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have ben associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described. (provided by RefSeq).
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-25388
controlid
SL1394
labexpid
SL2957,SL3254
replicate
1,2
softwareversion
MACS
antibody antibodydescription
Rabbit polyclonal IgG, epitope corresponding to amino acids 39-140 mapping near the N-terminus of ZEB1 of human origin.
antibody targetdescription
This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have ben associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described. (provided by RefSeq).
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-25388
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.2
protocol description
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
controlid
SL1394
labexpid
SL2957
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803411: HudsonAlpha_ChipSeq_GM12878_ZEB1_(SC-25388)_v041610.2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
47924244
Reads aligned (%)
40.3
Duplicates removed (%)
44.0
Number of peaks
8311 (qval < 1E-05)

hg38

Number of total reads
47924244
Reads aligned (%)
41.3
Duplicates removed (%)
43.9
Number of peaks
8099 (qval < 1E-05)

Base call quality data from DBCLS SRA