Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FOXA1

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
T-47D
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
epithelial cell line derived from a mammary ductal carcinoma.
antibody antibodydescription
Goat polyclonal IgG, epitope mapping at the C-terminus of FOXA1 of human origin. Antibody Target: FOXA1
antibody targetdescription
This gene encodes a member of the forehead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver (RefSeq).
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-6553
treatment description
1 h with 0.02% Dimethyl sufloxide (DMSO) (Myers)
controlid
SL1881
labexpid
SL2081,SL1878
replicate
1,2
softwareversion
MACS
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-6553
treatment
DMSO_0.02pct
treatment description
1 h with 0.02% DMSO (Myers)
protocol
v041610.2
protocol description
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
controlid
SL1881
labexpid
SL1878
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803409: HudsonAlpha_ChipSeq_T-47D_FOXA1_(SC-6553)_DMSO_0.02pct_v041610.2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
43925212
Reads aligned (%)
81.4
Duplicates removed (%)
11.5
Number of peaks
76842 (qval < 1E-05)

hg38

Number of total reads
43925212
Reads aligned (%)
82.9
Duplicates removed (%)
10.3
Number of peaks
76914 (qval < 1E-05)

Base call quality data from DBCLS SRA