Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Blood

Cell type

GM12891

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstein-Barr Virus transformed

Sample

source_name

GM12891

biomaterial_provider

Coriell

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell description

B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstein-Barr Virus transformed

antibody antibodydescription

mouse monoclonal, clone 8WG16. Antibody Target: POL2

antibody targetdescription

RNA Polymerase II

antibody vendorname

Covance

antibody vendorid

MMS-126R

protocol description

one 15-cycle round of PCR (Myers)

controlid

SL817

labexpid

SL908,SL921

labversion

SL908,SL921

replicate

1,2

softwareversion

MACS

antibody vendorname

Covance

antibody vendorid

MMS-126R

treatment

None

treatment description

No special treatment or protocol applies

protocol

PCR1x

protocol description

1-cycle of PCR (Myers)

controlid

SL817

labexpid

SL908

replicate

1

replicate description

tier 1

Sequenced DNA Library

library_name

GSM803399: HudsonAlpha_ChipSeq_GM12891_Pol2_PCR1x

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

52541912

Reads aligned (%)

80.2

Duplicates removed (%)

8.0

Number of peaks

25201 (qval < 1E-05)

hg19

Number of total reads

52541912

Reads aligned (%)

78.2

Duplicates removed (%)

7.4

Number of peaks

26075 (qval < 1E-05)