Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SPI1

Cell type

Cell type Class
Blood
Cell type
GM12891
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstein-Barr Virus transformed

Attributes by original data submitter

Sample

source_name
GM12891
biomaterial_provider
Coriell
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstein-Barr Virus transformed
antibody antibodydescription
Rabbit Polyclonal IgG. Antibody Target: PU1
antibody targetdescription
PU.1 (H-135)
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-22805
protocol description
one 15-cycle round of PCR (Myers)
controlid
SL817
labexpid
SL977,SL948
labversion
SL977,SL948
replicate
1,2
softwareversion
MACS
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-22805
treatment
None
treatment description
No special treatment or protocol applies
protocol
PCR1x
protocol description
1-cycle of PCR (Myers)
controlid
SL817
labexpid
SL977
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803398: HudsonAlpha_ChipSeq_GM12891_PU.1_PCR1x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
65392754
Reads aligned (%)
60.4
Duplicates removed (%)
15.5
Number of peaks
67108 (qval < 1E-05)

hg38

Number of total reads
65392754
Reads aligned (%)
61.9
Duplicates removed (%)
15.4
Number of peaks
64791 (qval < 1E-05)

Base call quality data from DBCLS SRA