Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BCL11A

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
H1-hESC
biomaterial_provider
WiCell Research Institute
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Mouse monoclonal [18B12DE6] to Ctip1. Raised against a cocktail of BCL11A fusion proteins. Reacts with human and mouse. Tested for ICC/IF, IHC-P, IP, WB. Antibody Target: BCL11A
antibody targetdescription
This gene encodes a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. Functions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. An essential factor in lymphopoiesis,is required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1 (By similarity)
antibody vendorname
abcam
antibody vendorid
ab19489
protocol description
one 15-cycle round of PCR (Myers)
controlid
SL1398
labexpid
SL1314
cell sex
M
antibody
BCL11A
antibody antibodydescription
Mouse monoclonal [18B12DE6] to Ctip1. Raised against a cocktail of BCL11A fusion proteins. Reacts with human and mouse. Tested for ICC/IF, IHC-P, IP, WB
antibody targetdescription
This gene encodes a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. Functions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. An essential factor in lymphopoiesis,is required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1 (By similarity)
antibody vendorname
Abcam
antibody vendorid
ab19489
treatment
None
treatment description
No special treatment or protocol applies
protocol
PCR1x
protocol description
1-cycle of PCR (Myers)
controlid
SL1398
labexpid
SL1314
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803396: HudsonAlpha_ChipSeq_H1-hESC_BCL11A_PCR1x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
29558771
Reads aligned (%)
71.8
Duplicates removed (%)
17.5
Number of peaks
1747 (qval < 1E-05)

hg19

Number of total reads
29558771
Reads aligned (%)
71.2
Duplicates removed (%)
18.6
Number of peaks
1411 (qval < 1E-05)

Base call quality data from DBCLS SRA