Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ZBTB33

Cell type

Cell type Class
Blood
Cell type
GM12878
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Attributes by original data submitter

Sample

source_name
GM12878
biomaterial_provider
GM12878
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus
antibody antibodydescription
Kaiso (6F8) Mouse monoclonal IgG1. Raised against amino acids 1-504 representing full length Kaiso of human origin. Recommended for detection of Kaiso of m, r, h, canine and chicken origin by WB, IP, IF and ELISA. Antibody Target: ZBTB33
antibody targetdescription
KAISO is a transcriptional regulator that binds, via its zinc fingers, to DNA sequences containing methylated CGCG or to the consensus KAISO-binding site (KBS) TCCTGCNA
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-23871
protocol description
one 15-cycle round of PCR (Myers)
controlid
SL516,SL517
labexpid
SL814,SL923
labversion
8,7
replicate
1,2
softwareversion
MACS
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-23871
treatment
None
treatment description
No special treatment or protocol applies
protocol
PCR1x
protocol description
1-cycle of PCR (Myers)
controlid
SL516
labexpid
SL814
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803392: HudsonAlpha_ChipSeq_GM12878_ZBTB33_PCR1x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
46129277
Reads aligned (%)
74.7
Duplicates removed (%)
4.4
Number of peaks
3028 (qval < 1E-05)

hg38

Number of total reads
46129277
Reads aligned (%)
76.7
Duplicates removed (%)
3.8
Number of peaks
2780 (qval < 1E-05)

Base call quality data from DBCLS SRA