Antigen

Antigen Class

TFs and others

Antigen

ZBTB33

Cell type

Cell type Class

Blood

Cell type

GM12878

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Sample

source_name

GM12878

biomaterial_provider

GM12878

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

antibody antibodydescription

Kaiso (6F8) Mouse monoclonal IgG1. Raised against amino acids 1-504 representing full length Kaiso of human origin. Recommended for detection of Kaiso of m, r, h, canine and chicken origin by WB, IP, IF and ELISA. Antibody Target: ZBTB33

antibody targetdescription

KAISO is a transcriptional regulator that binds, via its zinc fingers, to DNA sequences containing methylated CGCG or to the consensus KAISO-binding site (KBS) TCCTGCNA

antibody vendorname

Santa Cruz Biotechnology

antibody vendorid

sc-23871

protocol description

one 15-cycle round of PCR (Myers)

controlid

SL516,SL517

labexpid

SL814,SL923

labversion

8,7

replicate

1,2

softwareversion

MACS

antibody vendorname

Santa Cruz Biotech

antibody vendorid

sc-23871

treatment

None

treatment description

No special treatment or protocol applies

protocol

PCR1x

protocol description

1-cycle of PCR (Myers)

controlid

SL516

labexpid

SL814

replicate

1

replicate description

tier 1

Sequenced DNA Library

library_name

GSM803392: HudsonAlpha_ChipSeq_GM12878_ZBTB33_PCR1x

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

46129277

Reads aligned (%)

79.7

Duplicates removed (%)

3.8

Number of peaks

2780 (qval < 1E-05)

hg19

Number of total reads

46129277

Reads aligned (%)

78.2

Duplicates removed (%)

4.4

Number of peaks

3028 (qval < 1E-05)