Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
PAX5

Cell type

Cell type Class
Blood
Cell type
GM12878
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Attributes by original data submitter

Sample

source_name
GM12878
biomaterial_provider
GM12878
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus
antibody antibodydescription
Goat polyclonal IgG, 200 micrograms/ml. Epitope mapping at the C-terminus of Pax-5 of human origin. Recommended for detection of Pax-5 of m, r and h origin by WB, IP, IF, IHC(P) and ELISA. Antibody Target: PAX5
antibody targetdescription
This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation.
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-1974
protocol description
one 15-cycle round of PCR (Myers)
controlid
SL678,SL517
labexpid
SL735,SL675
labversion
5,6
replicate
1,2
softwareversion
MACS
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-1974
treatment
None
treatment description
No special treatment or protocol applies
protocol
PCR1x
protocol description
1-cycle of PCR (Myers)
controlid
SL678
labexpid
SL675
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803391: HudsonAlpha_ChipSeq_GM12878_PAX5-C20_PCR1x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
57549390
Reads aligned (%)
54.2
Duplicates removed (%)
14.1
Number of peaks
30979 (qval < 1E-05)

hg38

Number of total reads
57549390
Reads aligned (%)
55.4
Duplicates removed (%)
13.6
Number of peaks
30233 (qval < 1E-05)

Base call quality data from DBCLS SRA