Antigen

Antigen Class

TFs and others

Antigen

TCF12

Cell type

Cell type Class

Blood

Cell type

GM12878

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Sample

source_name

GM12878

biomaterial_provider

GM12878

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

antibody antibodydescription

HEB (A-20) Rabbit polyclonal IgG, 200 ?g/ml. Epitope mapping within the C-terminus of HEB of human origin. Antibody Target: TCF12

antibody targetdescription

The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

antibody vendorname

Santa Cruz Biotechnology

antibody vendorid

sc-357

protocol description

one 15-cycle round of PCR (Myers)

controlid

SL516,SL517

labexpid

SL673,SL1019

labversion

SL673

replicate

1,2

softwareversion

MACS

antibody vendorname

Santa Cruz Biotech

antibody vendorid

sc-357

treatment

None

treatment description

No special treatment or protocol applies

protocol

PCR1x

protocol description

1-cycle of PCR (Myers)

controlid

SL516

labexpid

SL673

replicate

1

replicate description

tier 1

Sequenced DNA Library

library_name

GSM803389: HudsonAlpha_ChipSeq_GM12878_TCF12_PCR1x

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

51915511

Reads aligned (%)

74.4

Duplicates removed (%)

11.7

Number of peaks

29971 (qval < 1E-05)

hg19

Number of total reads

51915511

Reads aligned (%)

73.4

Duplicates removed (%)

12.3

Number of peaks

30806 (qval < 1E-05)