Antigen

Antigen Class

TFs and others

Antigen

EBF1

Cell type

Cell type Class

Blood

Cell type

GM12878

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Sample

source_name

GM12878

biomaterial_provider

GM12878

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

antibody antibodydescription

Mouse monoclonal IgG, raised against a.a. 1-300 mapping at the N-terminus of EBF of human origin. Antibody Target: EBF1

antibody targetdescription

Early B-cell factor 1. Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3' (By similarity).

antibody vendorname

Santa Cruz Biotechnology

antibody vendorid

sc-137065

protocol description

one 15-cycle round of PCR (Myers)

controlid

SL516,SL517

labexpid

SL988,SL745

labversion

8,6

replicate

1,2

softwareversion

MACS

antibody vendorname

Santa Cruz Biotech

antibody vendorid

sc-137065

treatment

None

treatment description

No special treatment or protocol applies

protocol

PCR1x

protocol description

1-cycle of PCR (Myers)

controlid

SL516

labexpid

SL745

replicate

1

replicate description

tier 1

Sequenced DNA Library

library_name

GSM803386: HudsonAlpha_ChipSeq_GM12878_EBF1_(SC-137065)_PCR1x

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

71485533

Reads aligned (%)

70.0

Duplicates removed (%)

9.9

Number of peaks

49791 (qval < 1E-05)

hg19

Number of total reads

71485533

Reads aligned (%)

68.7

Duplicates removed (%)

10.2

Number of peaks

51937 (qval < 1E-05)