Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EBF1

Cell type

Cell type Class
Blood
Cell type
GM12878
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Attributes by original data submitter

Sample

source_name
GM12878
biomaterial_provider
GM12878
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus
antibody antibodydescription
Mouse monoclonal IgG, raised against a.a. 1-300 mapping at the N-terminus of EBF of human origin. Antibody Target: EBF1
antibody targetdescription
Early B-cell factor 1. Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3' (By similarity).
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-137065
protocol description
one 15-cycle round of PCR (Myers)
controlid
SL516,SL517
labexpid
SL988,SL745
labversion
8,6
replicate
1,2
softwareversion
MACS
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-137065
treatment
None
treatment description
No special treatment or protocol applies
protocol
PCR1x
protocol description
1-cycle of PCR (Myers)
controlid
SL516
labexpid
SL745
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803386: HudsonAlpha_ChipSeq_GM12878_EBF1_(SC-137065)_PCR1x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
71485533
Reads aligned (%)
61.7
Duplicates removed (%)
10.2
Number of peaks
51937 (qval < 1E-05)

hg38

Number of total reads
71485533
Reads aligned (%)
63.0
Duplicates removed (%)
9.9
Number of peaks
49791 (qval < 1E-05)

Base call quality data from DBCLS SRA