Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
HEY1

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
lab
HudsonAlpha
lab description
Myers - Hudson Alpha Institute for Biotechnology
datatype
ChipSeq
datatype description
ChIP-Seq
cell
K562
cell organism
human
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype
cancer
cell lineage
mesoderm
cell sex
F
antibody
HEY1
antibody antibodydescription
HRT1 (C-20) Goat polyclonal IgG, 200 ?g/ml. Epitope mapping near the C-terminus of HRT1 of human origin
antibody targetdescription
This gene encodes a nuclear protein belonging to the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcriptional repressors. Expression of this gene is induced by the Notch and c-Jun signal transduction pathways. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternative splicing results in multiple transcript variants.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-16424
treatment
None
treatment description
No special treatment or protocol applies
protocol
PCR1x
protocol description
1-cycle of PCR (Myers)
controlid
SL518
labexpid
SL845
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803385: HudsonAlpha_ChipSeq_K562_HEY1_PCR1x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
47818475
Reads aligned (%)
71.8
Duplicates removed (%)
9.7
Number of peaks
26089 (qval < 1E-05)

hg38

Number of total reads
47818475
Reads aligned (%)
73.0
Duplicates removed (%)
9.4
Number of peaks
26030 (qval < 1E-05)

Base call quality data from DBCLS SRA