Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SPI1

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Rabbit Polyclonal IgG. Antibody Target: PU1
antibody targetdescription
PU.1 (H-135)
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-22805
protocol description
one 15-cycle round of PCR (Myers)
controlid
SL518,SL519
labexpid
SL705,SL646
labversion
4
replicate
1,2
softwareversion
MACS
antibody targetdescription
PU.1 (H-135)
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-22805
treatment
None
treatment description
No special treatment or protocol applies
protocol
PCR1x
protocol description
1-cycle of PCR (Myers)
controlid
SL518
labexpid
SL646
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803384: HudsonAlpha_ChipSeq_K562_PU.1_PCR1x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
53813125
Reads aligned (%)
62.8
Duplicates removed (%)
9.3
Number of peaks
46606 (qval < 1E-05)

hg38

Number of total reads
53813125
Reads aligned (%)
63.9
Duplicates removed (%)
8.3
Number of peaks
46644 (qval < 1E-05)

Base call quality data from DBCLS SRA