Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SIX5

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Six5 (K-20) Goat polyclonal IgG, 200?g/ml. Epitope mapping near the C-terminus of Six5 of human origin. Recommended for detection of Six5 of human origin by WB, IF and ELISA. Antibody Target: SIX5
antibody targetdescription
The protein encoded by this gene is a homeodomain-containing transcription factor that appears to function in the regulation of organogenesis. This gene is located downstream of the dystrophia myotonica-protein kinase gene. Mutations in this geneare a cause of branchiootorenal syndrome type 2.
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-55706
controlid
SL3980,SL2455
labexpid
SL3990,SL3924
replicate
1,2
softwareversion
MACS
antibody
SIX5
antibody antibodydescription
Six5 (K-20) Goat polyclonal IgG, 200?g/ml. Epitope mapping near the C-terminus of Six5 of human origin. Recommended for detection of Six5 of human origin by WB, IF and ELISA
antibody targetdescription
The protein encoded by this gene is a homeodomain-containing transcription factor that appears to function in the regulation of organogenesis. This gene is located downstream of the dystrophia myotonica-protein kinase gene. Mutations in this geneare a cause of branchiootorenal syndrome type 2.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-55706
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.1
protocol description
2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)
controlid
SL2455
labexpid
SL3924
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803378: HudsonAlpha_ChipSeq_K562_SIX5_v041610.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
42089070
Reads aligned (%)
79.0
Duplicates removed (%)
13.4
Number of peaks
3903 (qval < 1E-05)

hg38

Number of total reads
42089070
Reads aligned (%)
80.1
Duplicates removed (%)
12.7
Number of peaks
3879 (qval < 1E-05)

Base call quality data from DBCLS SRA