Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SP1

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
H1-hESC
biomaterial_provider
WiCell Research Institute
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Sp1(E-3) is a mouse monoclonal antibody raised against amino acids 121-345 mapping near the N-terminus of Sp1 ofhuman origin. Recommended for detection of Sp1 of m, r and h origin by WB, IP, IF and ELISA. Antibody Target: SP1
antibody targetdescription
SP1 is a transcription factor that binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. It can interact with G/C-rich motifs from the serotonin receptor promoter.
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-17824
protocol description
one 15-cycle round of PCR (Myers)
controlid
SL1398
labexpid
SL1318,SL1166
replicate
1,2
softwareversion
MACS
antibody antibodydescription
Sp1(E-3) is a mouse monoclonal antibody raised against amino acids 121-345 mapping near the N-terminus of Sp1 ofhuman origin. Recommended for detection of Sp1 of m, r and h origin by WB, IP, IF and ELISA.
antibody targetdescription
SP1 is a transcription factor that binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. It can interact with G/C-rich motifs from the serotonin receptor promoter.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-17824
treatment
None
treatment description
No special treatment or protocol applies
protocol
PCR1x
protocol description
1-cycle of PCR (Myers)
controlid
SL1398
labexpid
SL1166
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803377: HudsonAlpha_ChipSeq_H1-hESC_SP1_PCR1x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
48469823
Reads aligned (%)
74.6
Duplicates removed (%)
7.6
Number of peaks
33729 (qval < 1E-05)

hg38

Number of total reads
48469823
Reads aligned (%)
75.9
Duplicates removed (%)
6.6
Number of peaks
32973 (qval < 1E-05)

Base call quality data from DBCLS SRA