Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Neural
Cell type
PFSK-1
Primary Tissue
Brain
Tissue Diagnosis
Medulloblastoma

Attributes by original data submitter

Sample

source_name
PFSK-1
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
neuroectodermal cell line derived from a cerebral brain tumor, (PMID: 1316433)
protocol description
a 25-cycle round of PCR and an additional 15-cycle round of PCR after gel size selection (Myers)
controlid
SL150,SL16,SL412
labexpid
SL150,SL16,SL412
replicate
1,3,2
cell karyotype
cancer
cell lineage
ectoderm
cell sex
M
antibody
RevXlinkChromatin
treatment
None
treatment description
No special treatment or protocol applies
protocol
PCR2x
protocol description
2-cycles of PCR (Myers)
controlid
SL150
labexpid
SL150
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803376: HudsonAlpha_ChipSeq_PFSK-1_RevXlinkChromatin_PCR2x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
56870897
Reads aligned (%)
63.9
Duplicates removed (%)
7.6
Number of peaks
16226 (qval < 1E-05)

hg19

Number of total reads
56870897
Reads aligned (%)
63.5
Duplicates removed (%)
8.3
Number of peaks
15915 (qval < 1E-05)

Base call quality data from DBCLS SRA