Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NR3C1

Cell type

Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
A549
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
epithelial cell line derived from a lung carcinoma tissue. (PMID: 175022), "This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old caucasian male." - ATCC, newly promoted to tier 2: not in 2011 analysis
antibody antibodydescription
Rabbit Polyclonal. Antibody Target: GR
antibody targetdescription
Glucocorticoid Receptor
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-1003
treatment description
1 h with 0.02% Ethanol (Myers)
protocol description
a 25-cycle round of PCR and an additional 15-cycle round of PCR after gel size selection (Myers)
controlid
SL247,SL305
labexpid
SL247,SL305
replicate
1,2
antibody targetdescription
Glucocorticoid Receptor
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-1004
treatment
EtOH_0.02pct
treatment description
1 h with 0.02% EtOH (Myers)
protocol
PCR2x
protocol description
2-cycles of PCR (Myers)
controlid
SL247
labexpid
SL247
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803373: HudsonAlpha_ChipSeq_A549_GR_EtOH_0.02pct_PCR2x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
84326234
Reads aligned (%)
38.6
Duplicates removed (%)
7.8
Number of peaks
17196 (qval < 1E-05)

hg38

Number of total reads
84326234
Reads aligned (%)
39.2
Duplicates removed (%)
7.0
Number of peaks
16665 (qval < 1E-05)

Base call quality data from DBCLS SRA