GSM803371: HudsonAlpha ChipSeq A549 GR DEX 100nM PCR2x
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
NR3C1
Cell type
Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
A549
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
epithelial cell line derived from a lung carcinoma tissue. (PMID: 175022), "This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old caucasian male." - ATCC, newly promoted to tier 2: not in 2011 analysis
antibody antibodydescription
Rabbit Polyclonal. Antibody Target: GR
antibody targetdescription
Glucocorticoid Receptor
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-1003
treatment description
1 h with 100 nM Dexamethasone (Myers)
protocol description
a 25-cycle round of PCR and an additional 15-cycle round of PCR after gel size selection (Myers)