Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
REST

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
H1-hESC
biomaterial_provider
WiCell Research Institute
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Mouse monoclonal. Antibody Target: NRSF
antibody targetdescription
Neuron-restrictive silencer transcription factor
antibody vendorname
Dr. David Anderson at Caltech
antibody vendorid
(Made by Protein Expression Center: pec@caltech.edu)
controlid
SL969
labexpid
SL978,SL1053
replicate
1,2
cell sex
M
antibody
NRSF
antibody antibodydescription
Mouse monoclonal
antibody targetdescription
Neuron-restrictive silencer transcription factor
antibody vendorname
David Anderson, Caltech
antibody vendorid
(Made by Protein Expression Center: pec@caltech.edu)
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.2
protocol description
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
controlid
SL969
labexpid
SL978
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803365: HudsonAlpha_ChipSeq_H1-hESC_NRSF_v041610.2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
72669372
Reads aligned (%)
42.9
Duplicates removed (%)
21.7
Number of peaks
16635 (qval < 1E-05)

hg38

Number of total reads
72669372
Reads aligned (%)
43.7
Duplicates removed (%)
21.0
Number of peaks
16665 (qval < 1E-05)

Base call quality data from DBCLS SRA