Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NR3C1

Cell type

Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
A549
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
epithelial cell line derived from a lung carcinoma tissue. (PMID: 175022), "This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old caucasian male." - ATCC, newly promoted to tier 2: not in 2011 analysis
antibody antibodydescription
Rabbit Polyclonal. Antibody Target: GR
antibody targetdescription
Glucocorticoid Receptor
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-1003
treatment description
1 h with 50 nM Dexamethasone (Myers)
protocol description
one 15-cycle round of PCR (Myers)
controlid
SL879,SL878
labexpid
SL882,SL883
labversion
1,2
replicate
1,2
softwareversion
MACS
antibody vendorid
sc-1004
treatment
DEX_50nM
treatment description
1 h with 50 nM DEX (Myers)
protocol
PCR1x
protocol description
1-cycle of PCR (Myers)
controlid
SL878
labexpid
SL882
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803358: HudsonAlpha_ChipSeq_A549_GR_DEX_50nM_PCR1x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
47148340
Reads aligned (%)
79.4
Duplicates removed (%)
7.4
Number of peaks
29539 (qval < 1E-05)

hg38

Number of total reads
47148340
Reads aligned (%)
81.1
Duplicates removed (%)
6.1
Number of peaks
29621 (qval < 1E-05)

Base call quality data from DBCLS SRA