Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Blood

Cell type

GM12878

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Sample

source_name

GM12878

biomaterial_provider

GM12878

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

antibody antibodydescription

mouse monoclonal, clone 8WG16. Antibody Target: POL2

antibody targetdescription

RNA Polymerase II

antibody vendorname

Covance

antibody vendorid

MMS-126R

protocol description

a 25-cycle round of PCR and an additional 15-cycle round of PCR after gel size selection (Myers)

controlid

SL217,SL218

labexpid

SL209,SL207

labversion

PCR2x

labversion description

a 25-cycle round of PCR and an additional 15-cycle round of PCR after gel size selection (Myers)

replicate

1,2

softwareversion

MACS

antibody vendorid

MMS-126R

treatment

None

treatment description

No special treatment or protocol applies

protocol

PCR2x

protocol description

2-cycles of PCR (Myers)

controlid

SL217

labexpid

SL207

replicate

1

replicate description

tier 1

Sequenced DNA Library

library_name

GSM803355: HudsonAlpha_ChipSeq_GM12878_Pol2_PCR2x

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

118330502

Reads aligned (%)

56.1

Duplicates removed (%)

23.8

Number of peaks

52850 (qval < 1E-05)

hg19

Number of total reads

118330502

Reads aligned (%)

55.1

Duplicates removed (%)

23.1

Number of peaks

54477 (qval < 1E-05)