Antigen

Antigen Class

TFs and others

Antigen

USF1

Cell type

Cell type Class

Blood

Cell type

GM12878

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Sample

source_name

GM12878

biomaterial_provider

GM12878

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

antibody antibodydescription

Rabbit polyclonal, USF-1 (C-20). Antibody Target: USF1

antibody targetdescription

The ubiquitously expressed cellular upstream stimulatory factor (USF) consists of USF-1 and USF-2 polypeptides which independently exhibit site-specific DNA binding and are members of the c-Myc-related family of regulatory factors containing helix-loop-helix domains. USF also contains a leucine repeat that is required for efficient DNA binding. USF was originally identified as an up- stream stimulatory factor that binds the core sequence CACGTG in the adeno- virus late promoter. These findings, together with the demonstration of coop- erative interaction between USF and the initiator-binding protein, TFII-I, raises the possibility of a more general involvement of USF in transcriptional regula- tion. While expression of both USF-1 and USF-2 species is ubiquitous, different ratios of USF homo- and heterodimers are found in different cell types.

antibody vendorname

Santa Cruz Biotechnology

antibody vendorid

sc-229

protocol description

a 25-cycle round of PCR and an additional 15-cycle round of PCR after gel size selection (Myers)

controlid

SL217,SL218

labexpid

SL448,SL483

labversion

PCR2x

labversion description

a 25-cycle round of PCR and an additional 15-cycle round of PCR after gel size selection (Myers)

replicate

1,2

softwareversion

MACS

antibody vendorid

sc-229

treatment

None

treatment description

No special treatment or protocol applies

protocol

PCR2x

protocol description

2-cycles of PCR (Myers)

controlid

SL217

labexpid

SL448

replicate

1

replicate description

tier 1

Sequenced DNA Library

library_name

GSM803347: HudsonAlpha_ChipSeq_GM12878_USF-1_PCR2x

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

58662101

Reads aligned (%)

56.5

Duplicates removed (%)

20.2

Number of peaks

9378 (qval < 1E-05)

hg19

Number of total reads

58662101

Reads aligned (%)

51.3

Duplicates removed (%)

14.4

Number of peaks

9804 (qval < 1E-05)