Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
HDAC2

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
H1-hESC
biomaterial_provider
WiCell Research Institute
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Goat polyclonal IgG, epitope mapping at the C-terminus of HDAC2 of human origin. Antibody Target: HDAC2
antibody targetdescription
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. (provided by RefSeq)
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-6296
controlid
SL1398
labexpid
SL3454,SL3935
replicate
1,2
softwareversion
MACS
antibody
HDAC2_(SC-6296)
antibody antibodydescription
Goat polyclonal IgG, epitope mapping at the C-terminus of HDAC2 of human origin.
antibody targetdescription
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. (provided by RefSeq)
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-6296
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.2
protocol description
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
controlid
SL1398
labexpid
SL3935
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803345: HudsonAlpha_ChipSeq_H1-hESC_HDAC2_(SC-6296)_v041610.2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
66947976
Reads aligned (%)
69.4
Duplicates removed (%)
20.7
Number of peaks
13708 (qval < 1E-05)

hg38

Number of total reads
66947976
Reads aligned (%)
71.0
Duplicates removed (%)
19.4
Number of peaks
14490 (qval < 1E-05)

Base call quality data from DBCLS SRA