Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BCL3

Cell type

Cell type Class
Blood
Cell type
GM12878
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Attributes by original data submitter

Sample

source_name
GM12878
biomaterial_provider
GM12878
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus
antibody antibodydescription
Rabbit polyclonal. Bcl-3(C-14) is an affinity purified antibody raised against a peptide mapping at the C-terminus of Bcl-3 of human origin. Recommended for detection of Bcl-3 of mouse, rat and human origin by WB, IP, IF and ELISA. Antibody Target: BCL3
antibody targetdescription
This gene is a proto-oncogene candidate. It is identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. The protein encoded by this gene contains seven ankyrin repeats, which are most closely related to those found in I kappa B proteins. This protein functions as a transcriptional co-activator that activates through its association with NF-kappa B homodimers. The expression of this gene can be induced by NF-kappa B, which forms a part of the autoregulatory loop that controls the nuclear residence of p50 NF-kappa B. (Provided by RefSeq).
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-185
controlid
SL1613
labexpid
SL3931,SL3728
replicate
1,2
softwareversion
MACS
antibody antibodydescription
Bcl-3(C-14) is an affinity purified rabbit polyclonal antibody raised against a peptide mapping at the C-terminus of Bcl-3 of human origin. Recommended for detection of Bcl-3 of m, r and h origin by WB, IP, IF and ELISA.
antibody targetdescription
This gene is a proto-oncogene candidate. It is identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. The protein encoded by this gene contains seven ankyrin repeats, which are most closely related to those found in I kappa B proteins. This protein functions as a transcriptional co-activator that activates through its association with NF-kappa B homodimers. The expression of this gene can be induced by NF-kappa B, which forms a part of the autoregulatory loop that controls the nuclear residence of p50 NF-kappa B. (Provided by RefSeq).
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-185
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.1
protocol description
2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)
controlid
SL1613
labexpid
SL3728
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803342: HudsonAlpha_ChipSeq_GM12878_BCL3_v041610.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
66884656
Reads aligned (%)
46.7
Duplicates removed (%)
41.2
Number of peaks
24886 (qval < 1E-05)

hg38

Number of total reads
66884656
Reads aligned (%)
48.1
Duplicates removed (%)
44.5
Number of peaks
24415 (qval < 1E-05)

Base call quality data from DBCLS SRA