Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TCF12

Cell type

Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

source_name
HepG2
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
hepatocellular carcinoma
antibody antibodydescription
HEB (A-20) Rabbit polyclonal IgG, 200 ?g/ml. Epitope mapping within the C-terminus of HEB of human origin. Antibody Target: TCF12
antibody targetdescription
The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-357
protocol description
one 15-cycle round of PCR (Myers)
controlid
SL1400,SL1401
labexpid
SL1167,SL1060
replicate
1,2
softwareversion
MACS
antibody targetdescription
The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-357
treatment
None
treatment description
No special treatment or protocol applies
protocol
PCR1x
protocol description
1-cycle of PCR (Myers)
controlid
SL1400
labexpid
SL1060
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803336: HudsonAlpha_ChipSeq_HepG2_TCF12_PCR1x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
73551720
Reads aligned (%)
65.9
Duplicates removed (%)
16.4
Number of peaks
8764 (qval < 1E-05)

hg38

Number of total reads
73551720
Reads aligned (%)
66.9
Duplicates removed (%)
15.6
Number of peaks
8875 (qval < 1E-05)

Base call quality data from DBCLS SRA