Antigen

Antigen Class

TFs and others

Antigen

REST

Cell type

Cell type Class

Neural

Cell type

SK-N-SH

Primary Tissue

Brain

Tissue Diagnosis

Neuroblastoma

Sample

source_name

SK-N-SH

biomaterial_provider

ATCC

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell description

neuroblastoma, newly promoted to tier 2: not in 2011 analysis, the SK-N-SH line was established from a neuroblastoma in a 4 year old individual.

antibody antibodydescription

Mouse monoclonal. Antibody Target: NRSF

antibody targetdescription

Neuron-restrictive silencer transcription factor

antibody vendorname

Dr. David Anderson at Caltech

antibody vendorid

(Made by Protein Expression Center: pec@caltech.edu)

protocol description

a 25-cycle round of PCR and an additional 15-cycle round of PCR after gel size selection (Myers)

controlid

SL108,SL410

labexpid

SL83,SL409

replicate

1,2

softwareversion

MACS

antibody targetdescription

Neuron-restrictive silencer transcription factor

antibody vendorname

David Anderson, Caltech

antibody vendorid

(Made by Protein Expression Center: pec@caltech.edu)

treatment

None

treatment description

No special treatment or protocol applies

protocol

PCR2x

protocol description

2-cycles of PCR (Myers)

controlid

SL108

labexpid

SL83

replicate

1

replicate description

tier 1

Sequenced DNA Library

library_name

GSM803335: HudsonAlpha_ChipSeq_SK-N-SH_NRSF_PCR2x

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg19

Number of total reads

80466820

Reads aligned (%)

43.9

Duplicates removed (%)

8.7

Number of peaks

36889 (qval < 1E-05)

hg38

Number of total reads

80466820

Reads aligned (%)

44.2

Duplicates removed (%)

8.3

Number of peaks

37040 (qval < 1E-05)